When combining with the PI3K inhibitor, acupuncture-mediated improvements in PCOS-like symptoms, endometrial receptivity, the number of implantation sites, and endometrial angiogenesis were altered, indicating that the PI3K/AKT signaling pathway may be involved in the mechanism of acupuncture on rats with PCOS. This evidence concerns the gene AKT1 and polycystic ovary syndrome.