Since PI3K/AKT pathway promotion participated in the protection of acupuncture against PCOS in PCOS-like symptoms, implantation sites' number, endometrial receptivity, and endometrial angiogenesis, the regulation of acupuncture in PI3K/AKT pathway expression was further investigated in PCOS rats. The gene discussed is AKT1; the disease is polycystic ovary syndrome.