Combined with the PPI prediction data and previous studies, we concluded that Efnb2 might exert its protective effect against MCAO/R-induced brain injury by controlling Nrp2 internalization, and the Efnb2/Nrp2 and Pten/Nrp2 pathways might be implicated in the EA-mediated protective effect against MCAO/R-induced brain injury. The gene discussed is EFNB2; the disease is injury.