As shown in Figure 8A, the levels of 5-HT, GABA, and BDNF in the blood samples from the premature rats with cognitive impairment were significantly lower than that from the normal control group, while the serum levels of GR, CRH, IL-6, and TNF-α were significantly up-regulated in rats with cognitive problem. This evidence concerns the gene CRH and Cognitive impairment.