Phosphorylation of Ascl1 on six SP sites (S62, S88, S185, S189, S202, and S218) by proline-directed serine-threonine kinases (e.g., ERK, GSK3, and CDK1; Figure 1A) has so far only been demonstrated in transfected HEK293 (Li et al., 2014), neuroblastoma (Woods et al., 2022), and glioblastoma (Azzarelli et al., 2022) cells in vitro. The gene discussed is ASCL1; the disease is neuroblastoma.