ACTB and coronary artery disorder: To evaluate the association between ACTB methylation in the peripheral blood and CHD, the same amplicon as described by Liu et al. (22) harboring 11 distinguished CpG groups (labeled as ACTB_CpG_2.3, ACTB_CpG_4.5, ACTB_CpG_6, ACTB_CpG_7.8, ACTB_CpG_9.10, ACTB_CpG_11, ACTB_CpG_12, ACTB_CpG_14, ACTB_CpG_15.16, ACTB_CpG_17, and ACTB_CpG_18) were amplified and analyzed by Agena MALDI-TOF mass spectrometry in 281 patients with CHD and 272 CHD-free controls.