Though limited by the sample size and statistical analysis, we noticed that the methylation levels of ACTB_CpG_2.3, ACTB_CpG_7.8 and ACTB_CpG_9.10 were increased in the HFrEF cases comparing to the controls, indicating that the aberrant DNA methylation pattern may play a role in the progress of HF. This evidence concerns the gene ACTB and hydrops fetalis.