The methylation levels of six CpG sites (ACTB_CpG_2.3, ACTB_CpG_7.8, and ACTB_CpG_9.10) in the ACTB amplicon exhibited efficient discriminatory power to distinguish general CHD cases, male CHD cases, HF cases, and NYHA I&II CHD cases from controls [area under curve (AUC) = 0.75, 0.74, 0.73, and 0.77, respectively, logistic regression model b; Figure 2, Supplementary Table 14]. The gene discussed is ACTB; the disease is hydrops fetalis.