To investigate whether miR-613 regulated NPC radiotherapy by inhibiting DNMT3B/TIMP3 and the STAT1/FOXO1 pathway, we detected STAT1 and FOXO1 protein levels in NP-69, C666-1, and C666-1R cells, and discovered that STAT1 protein levels were promoted and FOXO1 protein levels were decreased in C666-1 and C666-1R cells, with C666-1R cells showing the more obvious alteration. Here, DNMT3B is linked to nasopharyngeal carcinoma.