Herein, the current study set out to elucidate the potential roles of miR-613 in NPC cell radiosensitivity, and our findings highlighted that miR-613 inhibited DNMT3B, reduced TIMP3 methylation, and increased TIMP3 protein level, thereby inhibiting the downstream STAT1/FOXO1 pathway and augmenting the radiosensitivity of NPC cells. Here, STAT1 is linked to nasopharyngeal carcinoma.