Through the verification of SOX2 and OCT4 expressions, we discovered that overexpression of miR-22 can reduce the levels of SOX2 and OCT4 through inhibiting MAX in CRC cells, indicating that the miR-22/MAX axis might downregulate SOX2 and OCT4 to affect the self-renewal ability of CRC cells. Here, POU5F1 is linked to colorectal carcinoma.