Next, we performed differentially expressed genes (DEGs) on the TCGA data by using the R package DESeq2, the DEGs results showed that 577 genes were upregulated and 839 genes were downregulated, with GPX8 (log2FC = 4.879635261, p = 4.17e-17) being one of the most significantly upregulated genes in GBM (Figure 2A). Here, GPX8 is linked to glioblastoma.