GSVA pathway analysis revealed differentially regulated cancer pathways between the three m5C-clusters, including KRAS-related pathways (MAPK-, mTOR-, EGF- and ERK-pathways), cell death pathways (TNFR1-, FAS- and death-pathways), and cancer-related pathways (Wnt-, Gleevec-, MET and CREB-pathways) (Supplementary Figure S3F). This evidence concerns the gene KRAS and cancer.