Based on our RNA-sequencing analysis, which revealed substantial alterations in genes regulating the cell cycle and checkpoint responses to double-strand DNA breaks (ATM, ATR, CHEK1, and CHEK2) when human T-ALL cells were exposed to the adipocyte secretome, we next assessed cell cycle progression in leukemia cells cultured in unconditioned media, SCM, and ACM. Here, CHEK2 is linked to acute lymphoblastic leukemia.