HDAC9 and acute lymphoblastic leukemia: To further assess genomic alterations induced by the adipocyte secretome and epigenetic modifying drugs, we performed a nucleosome protection assay, in which DNA was isolated from human T-ALL cells cultured in unconditioned media, SCM, ACM, or cells cultured with the histone demethylase inhibitor GSK-J4 or HDAC inhibitor vorinostat, treated with nuclease, and run on an agarose gel to elevate chromatin fragmentation in malignant T cells.