In conclusion, our analysis is the first to explore the expression difference of AGTRAP between pan-cancer samples and their normal tissues, analyzing the prognostic impact and potential mechanisms of AGTRAP and its related genes in different kinds of cancers and revealing the correlation between AGTRAP and TME, TMB, MSI, neoantigen, and immune-related genes, which can assist predicting immunotherapy responses. This evidence concerns the gene AGTRAP and cancer.