Given that PTTG1 showed the same decreased expression trend in NCI-H460 and BxPC3 after knockdown of PRR11 (Figure 3A, middle and right panels) and was involved in the cell cycle and sister chromatid separation events mentioned before, we speculated that PRR11 may be involved in the development of pan-cancer by regulating the expression of PTTG1. The Spearman correlation between PRR11 and PTTG1 was more than 0.3 in 16/33 types of cancer (with ≥200 samples) in TCGA (Supplementary Figure S7). This evidence concerns the gene PRR11 and cancer.