After qRT-PCR validation, the present study found the two regulatory networks, (hsa_circ_0000324/miR-675-5p/MAPK14 and hsa_circ_0000324/miR-675-5p/SYK) which may correlate with differentiation and proliferation of CD4+ T cells in T1DM. The gene discussed is MAPK14; the disease is type 1 diabetes mellitus.