The average ΔCT values of nectin-1 (nectin-1-ACTB) in the five hepatoma cell lines were 11.42 ± 0.176, 10.09 ± 0.087, 10.69 ± 0.200, 9.50 ± 0.217, and 10.34 ± 0.302, respectively (the abundance of gene expression was high in the cell when the ΔCT value was ≤12). The gene discussed is NECTIN1; the disease is hepatocellular carcinoma.