The results showed that DNMT3A WT 3′-UTR + miR-186 co-transfection group showed a sharp decrease relative luciferase activity in osteosarcoma, and DNMT3A WT 3′-UTR + miR-186 inhibitor co-transfection group showed an apparent increase compared with DNMT3A MUT 3′-UTR + miR-186 co-transfection group (Figure 4B). Here, DNMT3A is linked to osteosarcoma.