In this study, the M1 and M2 macrophage polarization markers CCR7 and CD206 were higher expression in human degenerated NP tissues, and M1CM exacerbate IDD by inhibiting cell proliferation, cell anabolism, and promoting pro-inflammatory mediator secretion, while M2CM showed protection effects by suppressing TNF-α-induced ECM degradation and inflammation reaction. The gene discussed is TNF; the disease is intervertebral disk degenerative disorder.