We found that 4-PBA significantly alleviated pancreatic injury assessing by histological examination and pathological score analysis; reduced serum amylase and lipase levels; and markedly decreased GRP78, sXBP1, CHOP, RIP3 and pMLKL levels in pancreatic tissue during L-Arg-induced AP (Figure 4A-E). This evidence concerns the gene DDIT3 and alkaline phosphatase measurement.