To investigate the relationship of ER stress and necroptosis in AP, pancreatic acinar cells were stimulated by 200 nM CCK in vitro, ER stress indicators such as GRP78, sXBP1 and CHOP, and necroptosis markers such as RIP3 and pMLKL were detected by western blotting. Here, HSPA5 is linked to alkaline phosphatase measurement.