Similar to our previous results (29), titration of poly(I:C) in fish cells reveals that zebrafish LGP2, either at a low dose or at a high dose, synergistically promotes IFN response by low concentrations of poly(I:C), but significantly inhibits IFN response by high concentrations of poly(I:C), indicating that zebrafish LGP2 might act as an essential activator of IFN response at the early phase of virus infection, but as a negative regulator at the late phase of viral infection, which has been verified by SVCV infection in fish cells (29). Here, DHX58 is linked to viral infectious disease.