In contrast, when the TM-Taq was used, only the cancer mutant DNA templates (matched) were amplified, whereas the same number of copies (1,000,000 copies for BRAF; 30,000 copies for EGFR) of the WT templates (mismatched) were not detectable (ΔCT >25.1, 20.2, 21.3, and 20.3 for four cancer mutations) (Figure 2). Here, EGFR is linked to cancer.