EGFR and cancer: In these assays, two different cancer genes, BRAF and EGFR, were examined by using WT and cancer mutant plasmid DNA templates [single nucleotide (BRAF V600E and EGFR L858R) as well as deletion/insertion (EGFR Ex19Del and EGFR Ex20Ins) variants] and primers specific to cancer mutations (Figure 2).