Compared to the ALS mice (Fig. 4b), the numbers of Gli-1 cells increased in ALS + PUR mice while decreasing significantly in ALS + CYC mice; meanwhile, p-AKT cells behaved the same as Gli-1 cells in ALS + PUR mice as well as in ALS + CYC mice (Fig. 4c). The gene discussed is GLI1; the disease is amyotrophic lateral sclerosis.