Lipidomic analysis of mono- and polyunsaturated FA composition by Gas Chromatography-Mass Spectrometry (GC-MS) between Elovl5-silenced breast cancer cells and their respective control breast cancer cells resulted in the accumulation of C16:1 n-7 and C18:3 n-6 FA (Supplementary Fig. S1H–J) whereas C16:1 n-7 and C18:3 n-6 content decreased in 4T1 cells stably overexpressing Elovl5 (Supplementary Fig. S1K). This evidence concerns the gene ELOVL5 and breast cancer.