TGFBR2 and breast carcinoma: We confirmed at the protein level that the expression of TGF-β receptor 1 (TGFβ-R1) by immunofluorescence staining (Fig. 4C) and expression of TGF-β receptor 2 (TGFβ-R2) by western blotting (Fig. 4D) were enhanced in Elovl5-depleted breast cancer cells.