ELOVL5 and breast carcinoma: We showed that the breast cancer cells (MCF-7 and 4T1) with depletion of Elovl5 expression increased their content of intracellular LD (green dots in the cells) detected by fluorescence microscopy using Bodipy 493/503 or Nile red staining (Fig. 5H and Supplementary Fig. S5B–D).