ENAH and cancer: Surprisingly, we did not observe any difference (p > 0.05; Mann–Whitney U-test) in TMEM Activity-MRI between MENA-WT and MENA-KO mice (Fig. 4e, f), implying that MENA is not directly involved in the regulation of TMEM doorway activity, but may exclusively contribute to the establishment of the proinvasive/promigratory cancer cell subpopulation that disseminates via TMEM doorways46–49.