FACS analysis using antibodies for LAG3, TIGIT, CTLA4 and PD1 indeed showed that more T cells significantly expressed these receptors compared with T cells obtained from spleens of wild-type mice (figure 6A), further demonstrating that anti-tumor response relying on T cells is impaired in the TH-MYCN mouse model. This evidence concerns the gene PDCD1 and neoplasm.