Multivariable analyses revealed significantly prognostic candidates of OS were lymph node metastasis (p < 0.001), distant metastasis (p < 0.001), pathologic cancer stage (p = 0.003) and pre-CCRT SPINK1 (p = 0.003) in RC patients (Table 3). The gene discussed is SPINK1; the disease is metastatic malignant neoplasm in the lymph nodes.