Taken together, our study supported that miR‐10b‐5p could be encapsulated by glioma cell‐derived EVs into macrophages and provided evidence suggesting that hypoxic glioma cell‐derived EVs carrying miR‐10b‐5p could stimulate M2 polarization of macrophages and accelerate glioma cell proliferation, migration, and invasion through activated PI3K/AKT pathway by increasing expression of PIK3CA via downregulation of NEDD4L expression. Here, AKT1 is linked to central nervous system cancer.