SPC25 and neoplasm: Figure 4 shows survival analysis results of hub genes. They were VRK1, NUP37, HMMR, SPC25, and RUVBL1 and patients with high expression had lower overall survival. Furthermore, TCGA data showed the expression of these five genes in tumor tissues was significantly higher than that in normal tissues, and they were up-regulated in advanced tumor tissues (Figure 5). In addition, based on The Human Protein Atlas (HPA), the protein expression levels of these five genes were significantly higher in tumor tissues than in normal tissues (Supplementary Figure S1).