ROC analysis of sPD-L1 allowed significant differentiation between HC group and CRC group without lymph node metastasis (AUC=0.774, 95% CI 0.732-0.811, p<0.001), HC group and CRC group with lymph node metastasis (AUC=0.948, 95% CI 0.923-0.966, p<0.001), HC group and CRC group (lymph node metastasis and non lymph node metastasis, AUC=0.861, 95% CI 0.830-0.887, p<0.001) as showed in Figure 2. Here, SPDL1 is linked to metastatic malignant neoplasm in the lymph nodes.