The lack of detection of common CRC related genes including EGFR, KRAS, BRAF and p53 in tumor together with CEA, CA19-9 and CA24-2 in serum is the limitation of present study.Our future work will focus on the integration of sPD-L1 level in serum and PD-L1 expression on tumor cells in the separate cohorts to future investigate the roles of sPD-L1 on diagnosis for early detection of CRC, prediction for response to the therapy, risk of lymph node metastasis, and prognosis of patients. Here, EGFR is linked to metastatic malignant neoplasm in the lymph nodes.