The results suggested that the mRNA expression of EFNA1, EFNA3 and EFNA4 was evidently higher in five HCC cell lines (HCC-LM3, MHCC97-H, SMMC 7721, Huh-7, and HepG2) (p < 0.05) (Figures 11A,C,D) and tumor tissues (p = 0.0362, p = 0.0021 and p = 0.0032, respectively) (Figures 11I,K,L) than in a normal liver cell line (L-02) and paired para-cancerous tissues. The gene discussed is EFNA4; the disease is hepatocellular carcinoma.