To further discuss the potential correlation between ephrin genes and the tumor immune microenvironment, we applied the ESTIMATE algorithm to calculate immune and stromal scores for each HCC sample and then analyzed the association of ephrin genes (EFNA3, EFNA4, and EFNB1) with immune scores, stromal scores, ESTIMATE scores, and tumor purity by using the Spearman correlation method. The gene discussed is EFNA4; the disease is neoplasm.