In the above bioinformatics analysis based on the TCGA dataset, we found that some ephrin genes (EFNA1, EFNA3, EFNA4, EFNB1, EFNB2) were significantly upregulated in HCC tissues, and EFNB3 was downregulated in HCC tissues, while EFNA2 and EFNA5 showed no significant differences between cancer tissues and adjacent normal tissues. The gene discussed is EFNA4; the disease is cancer.