We found that the expression of EFNA1, EFNA3, EFNA4, EFNB1, and EFNB2 was significantly higher in HCC tissues than in paired normal tissues, and higher expression of EFNA1, EFNA3, EFNA4, EFNA5, and EFNB1 was associated with worse overall survival in patients with HCC. Here, EFNB1 is linked to hepatocellular carcinoma.