We found that the expression of EFNA1, EFNA3, EFNA4, EFNB1, and EFNB2 was significantly higher in HCC tissues than in paired normal tissues, and higher expression of EFNA1, EFNA3, EFNA4, EFNA5, and EFNB1 was associated with worse overall survival in patients with HCC. This evidence concerns the gene EFNA5 and hepatocellular carcinoma.