We have previously established Nestin-CreERT2QkL/LPtenL/LTrp53L/L (QPP) cohort and demonstrated that QPP mice injected with tamoxifen at postnatal day 7 (P7) developed GBM with a penetrance of over 90% and died with a median survival time of ∼105 days, whereas Nestin-CreERT2PtenL/LTrp53L/L (PP) cohort did not develop GBM (Shingu et al., 2017). This evidence concerns the gene NES and glioblastoma.