NES and glioblastoma: To test whether Prkn deletion could also promote GBM development in the backdrop of Pten/Trp53 double knockout, we crossed Prkn-null allele to Nestin-CreERT2PtenL/LTrp53L/L (PP) mice to generate Nestin-CreERT2PtenL/LTrp53L/LPrkn−/− (PPP) cohort (Figure 1A).