PLAs including ones for VAPB and PTPIP51 have already been used to quantify ER-mitochondria contacts and signaling in models of ALS, Parkinson’s disease and Alzheimer’s disease (De Vos et al., 2012; Hedskog et al., 2013; Bernard-Marissal et al., 2015; Stoica et al., 2016; Paillusson et al., 2017; Gomez-Suaga et al., 2019; Gomez-Suaga et al., 2022). Here, RMDN3 is linked to early-onset autosomal dominant Alzheimer disease.