A previous study has found that PD-L1 is an independent prognostic factor in patients with liver cancer, and the high expression of PD-L1 significantly correlates with the high Edmondson grade (P < 0.01) [23].The N-ethylmaleimide-sensitive fusion protein attachment protein receptor YKT6 and pregnancy upregulated nonubiquitous calmodulin kinase (PNCK) are closely related to the progression HCC, which may be used as potential biomarkers of a poor prognosis in patients with HCC. The gene discussed is YKT6; the disease is hepatocellular carcinoma.