At last, CKD mice were injected with the corresponding AAV (sh-NC + Vector, sh-Drp1 + Vector, and sh-Drp1 + EZH2) to explore whether Drp1 could regulate the ROS/HIF-1α/EZH2 axis to alleviate neurological impairment induced by hypercalcemia in CKD. Here, EZH2 is linked to chronic kidney disease.