Protein levels of CD163 were significantly higher in HGG compared to LGG (Mann–Whitney, p = 0.008, Fig. 3b), and more HGG had any staining compared to LGG (Fisher’s exact, p = 0.0023), indicating the presence of a higher immune infiltrate in the more aggressive glioblastomas, as described previously35. Here, CD163 is linked to glioblastoma.