At lower concentrations (5–10 μM), Compound 5a doubled (i.e., 10 vs. 5 μM) the percentage of apoptotic CRC cells, which aligned with Compound 5a-induced p53 upregulation, tumor suppressor protein well described to mediate apoptosis (Marei et al., 2021). The gene discussed is TCHP; the disease is colorectal carcinoma.