Finally, to ask whether M2-CM influences TMZ resistance in a PI3K/Akt/Nrf2 pathway-dependent manner, we performed FCM assay and found that the rescuing effects of M2-CM on TMZ-induced GBM cell apoptosis were abolished by both LY294002 and Nrf2 knockdown (Figures 7(a) and 7(b)). Here, AKT1 is linked to glioblastoma.