To investigate this issue, the GBM cells were cocultured with M2-CM with or without VEGF deletion, and the Western Blot analysis results showed that M2-CM increased the expression levels of phosphorylated PI3K (p-PI3K) and Akt (p-Akt) in the GBM cells, which were suppressed by silencing VEGF (Figures 5(a)–5(e)). This evidence concerns the gene VEGFA and glioblastoma.