At the same time, the relationship between PDIA3 promoter region methylation and patient outcome was further analyzed, and it is found that PDIA3 promoter region methylation level was a protective factor for survival in some tumors, especially in BLCA, and PDIA3 promoter region methylation level was positively correlated with OS, DSS, and PFI. This evidence concerns the gene PDIA3 and bladder transitional cell carcinoma.