The results obtained by western blotting of LC3B showed that the LC3B-II/LC3B-I ratio in ALS astrocytes was significantly lower than that in control subject astrocytes (Figures 3(i) and 3(j)), and the relative level of the p62 protein in ALS astrocytes was significantly higher than that in control subject astrocytes (Figure 3(i) and 3(k)). This evidence concerns the gene MAP1LC3B and amyotrophic lateral sclerosis.