Increased secretion of TGFβ1 and VEGF of CM obtained from 3D cultures compared with baseline culture medium (αMEM). VEGF: 1,015.17 ± 170.97 [pg/ml], TGFβ1: 14.33 ± 6.71 [pg/ml]. Increased in vitro pro-angiogenic effects of CM obtained from 3 days cultures confirmed by a tube-formation assay. The continuous infusion of CM obtained from 3D cultures induced significantly better functional and structural recovery after stroke, reducing the infarction volume and maintained motor function in an ischemic stroke model. The gene discussed is TGFB1; the disease is stroke disorder.