According to flow cytometry analysis, the mean fluorescence intensity (MFI) of PD-L1 decreased significantly in the Lv-BIRC5 group rather than the Lv-NC group, as shown in Figure 6C. Since the PD-1/PD-L1 signaling pathway axis modulates an allosteric immune response, we wondered if BIRC5 overexpression in lung cancer cells would upregulate PD-L1 and encourage immune escape. This evidence concerns the gene CD274 and lung cancer.