Meanwhile, the mRNA and protein levels of ATAD2 and KIF4A in EC109 and KYSE30 cells stimulated with different concentrations of aspirin for 48 h were both decreased, which suggested that aspirin might exert its antitumor activity in ESCC by inhibiting the expression of ATAD2 and KIF4A. Here, ATAD2 is linked to esophageal squamous cell carcinoma.