This correlated with a significant reduction in the frequency of double positive (DP) CD4+CD8+ thymocytes (37.4% reduction compared to TcdA−TcdB−CDT+-infection and a 38.1% reduction when compared to uninfected mice; p= .0079; Figure 2g) with a reciprocal increase in the frequency of single positive (SP) CD4+ and CD8+ T-cell populations following WT-infection compared to the TcdA−TcdB−CDT+-infected mice (14.5% and 16.44% proportional shift, respectively) and uninfected mice (15.04% and 16.51% proportional shift, respectively). This evidence concerns the gene CD8A and infection.