To gain further insights into the effect and mechanism of sEVs‐GFAT1 in ECs in the TME, we established a series of in vitro models to simulate the TME and validate whether sEVs‐GFAT1 heighten HBP‐mediated O‐GlcNAcylation in ECs and thus promote tumor angiogenesis (Figure S4E, Supporting Information). This evidence concerns the gene GFPT1 and neoplasm.