TDP-43 is a DNA/RNA binding protein which translocates to the cytoplasm and disrupts RNA metabolism in the majority of ALS patients.16,17 To test whether this also occurred in our disease model, the number of ChAT+ motor neurons expressing TDP-43 exclusively in the cytoplasm was quantified in the cervical spinal cord. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.