PI3K-Akt signaling plays an important role in many diseases, including cancer, diabetes, obesity, cardiovascular diseases, and neurological diseases (depression, anxiety).[29–31] Cao et al[23] reported that icariin significantly ameliorates the seeming symptoms in rats with perimenopausal depression depending on the regulation of PI3K-Akt signaling. This evidence concerns the gene AKT1 and obesity due to melanocortin 4 receptor deficiency.