Ranking by P value, the top 20 pathways are shown in Figure 4B. The first 10 signaling pathways were proteoglycans in cancer (hsa05205), pathways in cancer (hsa05200), prostate cancer (hsa05215), hypoxia-inducible factor-1 (HIF-1; hsa04066), central carbon metabolism in cancer (hsa05230), estrogen (hsa04915), PI3K-Akt (hsa04151), ErbB (hsa04012), Rap1 (hsa04015), and prolactin (hsa04917). The gene discussed is HIF1A; the disease is prostate cancer.