SLC9A3 and colonic neoplasm: To confirm decreased NHE3 expression in an independent cohort of CRC patients, we tested paired biopsy samples from unaffected colon tissues and colorectal cancer for expression of NHE3 using an array plate with normalized cDNA from 24 matched biopsy samples from human colon cancer and unaffected sites, covering 24 normal, 5 stage I, 5 stage IIA, 2 stage IIB, 2 stage IIIA, 3 stage IIIB, 2 stage IIIC, 1 stage III, and 4 stage IV samples (OriGene).