To determine the biological functions of GASP1 in breast cancer, we ectopically expressed GASP1 in MDA-MB-231 and DU4475 cells by a lentivirus-mediated system (Fig. 2a) and found that GASP1 overexpression significantly promoted cell proliferation and clone formation compared with the control (Fig. 2b, c). The gene discussed is GPRASP1; the disease is breast carcinoma.