In a separate data set of over 1,000 pediatric high-grade gliomas, including DMGs, we found VRK2 promoter methylation in subsets of histone 3 wild-type and H3 K27M tumors, but VRK2 promoter methylation was most highly associated with the histone H3 G34R mutation (Figure 2D). This evidence concerns the gene VRK2 and central nervous system cancer.