VRK2 and glioma: In a separate data set of over 1,000 pediatric high-grade gliomas, including DMGs, we found VRK2 promoter methylation in subsets of histone 3 wild-type and H3 K27M tumors, but VRK2 promoter methylation was most highly associated with the histone H3 G34R mutation (Figure 2D).