To determine whether the reduced viability was due to apoptosis, we performed live-cell experiments with a caspase-3/7 (CASP3/7) cleavage reporter in the LN443 GBM cell line and found significantly higher CASP3/7 activity after VRK1 KO (Figure 1F and Supplemental Figure 1D). This evidence concerns the gene CASP3 and glioblastoma.