Three of the 4 VRK1 sgRNAs that were included in the DepMap led to robust VRK1 KO and proliferation defects in NB, DMG, and glioblastoma multiforme (GBM) cultures (Figure 1C and Supplemental Figure 1, A–C; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.158755DS1). Here, VRK1 is linked to glioblastoma.