Further analysis of the cytokine and chemokine expression profile showed the elevation in IL6, TNFα, and CCL5 mRNA levels in late passages, and it was particularly exacerbated in DM1 fibroblasts (Figure 5I), providing additional evidence of the accumulation of senescent cells and the acquisition of the SASP in DM1 fibroblasts. This evidence concerns the gene TNF and myotonic dystrophy type 1.