Importantly, MITF immunostaining of SOX10+ and SOX10– primary tumors from NSG mice detected a subpopulation of MITF+ melanoma cells in primary tumors from NSG mice injected with SOX10+MITF– melanoma cells, while such melanoma cells were not found in SOX10– NSG primary tumors (Figure 4A). This evidence concerns the gene MITF and melanoma.