In order to discern the role of SOX10 in determining the aggressive phenotype of MITF-methylated melanoma, we specifically targeted SOX10 by CRISPR/Cas9 to generate a SOX10 knockout (SOX10KO) MITF-methylated melanoma cell line with SOX10-unmethylated promoter CpGs (Figure 6). The gene discussed is SOX10; the disease is melanoma.