The observation indicated that the levels of anti‐B2GPI and ACA in the pcDNA3.1‐SAMD1 group were markedly lower relative to the pcDNA3.1‐NC group (Figure 4B,C, p < .05), and the levels of anti‐B2GPI and ACA in the pcDNA3.1‐NC group were not significantly different from those in the APS group. This evidence concerns the gene SAMD1 and autoimmune polyendocrinopathy.