The transwell assay demonstrated that the number of migrating cells in the pcDNA3.1‐SAMD1 group was increased versus the pcDNA3.1‐NC group (Figure 4I, p < .05), and no marked difference was seen between the pcDNA3.1‐NC group and the APS group. This evidence concerns the gene SAMD1 and autoimmune polyendocrinopathy.