The results of qRT‐PCR and Western blot for measuring the mRNA and protein expressions of MMP‐9, iNOS, ICAM‐1, and MCP‐1 in HUVECs in the APS, pcDNA3.1‐NC, and pcDNA3.1‐SAMD1 groups showed that the mRNA and protein expressions of MMP‐9, iNOS, ICAM‐1, and MCP‐1 in HUVECs in the pcDNA3.1‐SAMD1 group were also significantly lower than those in the pcDNA3.1‐NC group (Figure 4D,E, p < .05). This evidence concerns the gene SAMD1 and autoimmune polyendocrinopathy.