The results exhibited that the positive rate of SA‐β‐gal in the pcDNA3.1‐SAMD1 group was lower versus the pcDNA3.1‐NC group (Figure 4F, p < .05), and there was no obvious difference between the pcDNA3.1‐NC group and the APS group. This evidence concerns the gene SAMD1 and autoimmune polyendocrinopathy.